Russell S R, Sanchez-Soriano N, Wright C R, Ashburner M
Department of Genetics, University of Cambridge, UK.
Development. 1996 Nov;122(11):3669-76. doi: 10.1242/dev.122.11.3669.
We have cloned and characterised a member of the High Mobility Group superfamily of genes from Drosophila, Sox70D, which is closely related to the mammalian testis determining gene SRY. Sox70D corresponds to the dominant wing mutation Dichaete. Homozygous deletions of the Sox70D gene and recessive lethal Dichaete alleles have a variable embryonic segmentation phenotype. Dichaete is expressed in early embryos in a dynamic pattern reminiscent of gap and pair-rule genes and is required for the appropriate expression of the primary pair-rule genes even skipped, hairy and runt. The molecular nature of Dichaete and its expression pattern during early embryogenesis suggest that the gene plays a key role in early development; the variability in both the segmentation phenotype and the effects on pair-rule gene expression suggests that this role is to support the transcriptional regulation of key developmental genes rather than directly regulate any one of them.
我们已经克隆并鉴定了果蝇高迁移率族基因超家族的一个成员Sox70D，它与哺乳动物的睾丸决定基因SRY密切相关。Sox70D对应显性翅突变“双胸”。Sox70D基因的纯合缺失和隐性致死“双胸”等位基因具有可变的胚胎分节表型。“双胸”在早期胚胎中以一种动态模式表达，让人联想到缺口基因和成对规则基因，并且是主要成对规则基因“偶数缺失”“毛状”和“矮胖”适当表达所必需的。“双胸”的分子特性及其在早期胚胎发生过程中的表达模式表明，该基因在早期发育中起关键作用；分节表型的变异性以及对成对规则基因表达的影响表明，这一作用是支持关键发育基因的转录调控，而不是直接调控其中任何一个基因。
