Fushimi H, Komatsu K, Isobe M, Namba T
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 1996 Nov;19(11):1530-2. doi: 10.1248/bpb.19.1530.
Total DNA was extracted from the fresh underground parts of three Panax separate species. The 18S rRNA regions of extracted DNA were amplified by the polymerase chain reaction (PCR) and their sequences were determined. In each species, the sequences were found to be of 1809 base pairs (bps) but with different gene sequences. Different base substitutions were observed at nucleotide positions 497, 499, 501 and 712. The same procedure was performed on commercial samples of Ginseng Radix, Panacis Japonici Rhizoma and American Ginseng. Each sequence completely corresponded with that of each original plant, namely P. ginseng, P. japonicus and P. quinquefolius, respectively. This is the first time that 18S rRNA gene sequencing on Panax species was carried out. Previously, Ginseng drugs have been identified mainly by their external and internal structure. Thus this method will be useful in identifying Ginseng drugs at the gene level.
从三种不同的人参属植物的新鲜地下部分提取总DNA。通过聚合酶链反应（PCR）扩增提取的DNA的18S rRNA区域，并测定其序列。在每个物种中，发现序列长度为1809个碱基对（bps），但基因序列不同。在核苷酸位置497、499、501和712处观察到不同的碱基替换。对人参、竹节参和西洋参的商业样品进行了相同的操作。每个序列分别与每种原始植物（即人参、竹节参和西洋参）的序列完全对应。这是首次对人参属植物进行18S rRNA基因测序。此前，人参类药物主要通过其外部和内部结构进行鉴定。因此，该方法将有助于在基因水平上鉴定人参类药物。
