Ngan F, Shaw P, But P, Wang J
Department of Biochemistry, Chinese University of Hong Kong, Shatin, People's Republic of China.
Phytochemistry. 1999 Mar;50(5):787-91. doi: 10.1016/s0031-9422(98)00606-2.
Using conserved plant sequences as primers, the DNA sequences in the ribosomal ITS1-5.8S-ITS2 region have been amplified and determined for six Panax species, P. ginseng C. A. Mey. (Oriental ginseng), P. quinquefolius L. (American ginseng), P. notoginseng (Burkill) F. H. Chen (Sanchi), P. japonicus C. A. Mey. (Japanese ginseng), P. trifolius L. and P. major Ting, as well as two common adulterants of ginseng, Mirabilis jalapa L. and Phytolacca acinosa Roxb. An authentication procedure based upon the restriction fragment length polymorphism (RFLP) in the region is able to differentiate between P. ginseng and P. quinquefolius, and to discriminate the ginsengs from the two common poisonous adulterants. Broader application of this approach to authenticate other morphologically similar Chinese medicinal materials is rationalised.
以保守的植物序列为引物，对六种人参属植物，即人参（C. A. Mey.）（东方人参）、西洋参（L.）（美国人参）、三七（Burkill）F. H. Chen（三七）、竹节参（C. A. Mey.）（日本人参）、三花（L.）和大叶三七（Ting），以及两种常见的人参掺假品紫茉莉（L.）和商陆（Roxb.）的核糖体ITS1 - 5.8S - ITS2区域的DNA序列进行了扩增和测定。基于该区域限制性片段长度多态性（RFLP）的鉴定程序能够区分人参和西洋参，并将人参与两种常见的有毒掺假品区分开来。将这种方法更广泛地应用于鉴定其他形态相似的中药材是合理的。
