Ang L C, Perry J R, Bilbao J M, Ozane W, Peschke E, Young B, Nelson N
Division of Pathology, Sunnybrook Health Science Center, Toronto, Canada.
Can J Neurol Sci. 1996 Nov;23(4):251-6. doi: 10.1017/s031716710003818x.
Thirty-one gliosarcomas (25 nonirradiated and 6 postirradiated tumors) were selected based on the presence of two distinctive areas: a malignant gliomatous and a sarcomatous component. In all cases, the sarcomatous component appears like fibrosarcoma or malignant fibrous histiocytoma. Two tumors showed additional areas consisting of osteochondroid differentiation.
All tumors were examined using antibodies against Ulex europaeus agglutinin I (UEA), glial fibrillary acidic protein (GFAP), vimentin (VM), epithelial membrane antigen (EMA), desmin, collagen IV, alpha-1-antitrypsin (alpha-1-AT) and smooth muscle actin (SMA).
While the VM highlighted the sarcomatous areas of all tumors there were only scattered spindle cells that were GFAP-positive in the same areas. The alpha-1-AT was diffusely reactive in the sarcomatous areas in 20 cases. Focal immunoreactivity was seen with SMA (20 tumors), UEA (8 tumors), EMA (5 tumors), collagen IV (5 tumors) and desmin (4 tumors) in the nonvascular sarcomatous cells.
The range of immunophenotypical expression is likely to be a reflection of the capacity of a multipotential progenitor to undergo divergent differentiation. There is a very little morphological difference between the postirradiated and nonirradiated tumors except that a higher proportion of postirradiated tumors are immunoreactive to SMA and desmin.
基于存在两个不同区域（恶性胶质瘤成分和肉瘤成分），选取了31例胶质肉瘤（25例未接受放疗的肿瘤和6例放疗后的肿瘤）。在所有病例中，肉瘤成分表现为纤维肉瘤或恶性纤维组织细胞瘤。2例肿瘤显示有由骨软骨样分化组成的额外区域。
使用抗欧洲荆豆凝集素I（UEA）、胶质纤维酸性蛋白（GFAP）、波形蛋白（VM）、上皮膜抗原（EMA）、结蛋白、IV型胶原、α1抗胰蛋白酶（α1-AT）和平滑肌肌动蛋白（SMA）的抗体对所有肿瘤进行检查。
虽然VM突出显示了所有肿瘤的肉瘤区域，但在相同区域只有散在的梭形细胞呈GFAP阳性。α1-AT在20例肿瘤的肉瘤区域呈弥漫性反应。在非血管性肉瘤细胞中，SMA（20例肿瘤）、UEA（8例肿瘤）、EMA（5例肿瘤）、IV型胶原（5例肿瘤）和结蛋白（4例肿瘤）可见局灶性免疫反应性。
免疫表型表达范围可能反映了多能祖细胞进行不同分化的能力。放疗后和未放疗的肿瘤之间形态学差异很小，只是放疗后肿瘤对SMA和结蛋白免疫反应性的比例更高。
