Likhtenshtein G I, Bishara R, Papper V, Uzan B, Fishov I, Gill D, Parola A H
Department of Chemistry, Ben Gurion University of Negev, Beer Sheva, Israel.
J Biochem Biophys Methods. 1996 Nov 15;33(2):117-33. doi: 10.1016/s0165-022x(96)00022-x.
A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180 degrees. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.
一种基于监测对位取代芪（PSS）光致变色光异构化的荧光参数和动力学的新型光致变色荧光方法（PFLM）已被提出。结果表明，PSS表现出与典型膜荧光探针如二苯基己三烯（DPH）相似的荧光特性。对PSS反式-顺式和顺式-反式光异构化动力学的研究使得在特定条件下能够估计PSS激发态下芪片段在180度固定角度时的旋转相关时间。在粘性介质中，这个过程是速率决定阶段。总之，荧光和光致变色这两种技术都能够建立详细的机制并测量芪探针（PSS）在膜中迁移的定量参数。PFLM被应用于大肠杆菌膜动力学的研究。
