透明质酸钠对兔角膜上皮细胞体外生长的影响。

The effect of sodium hyaluronate on the growth of rabbit corneal epithelial cells in vitro.

作者信息

Miyazaki T, Miyauchi S, Nakamura T, Takeshita S, Horie K

机构信息

Tokyo Research Institute, Seikagaku Corporation, Japan.

出版信息

J Ocul Pharmacol Ther. 1996 Winter;12(4):409-15. doi: 10.1089/jop.1996.12.409.

DOI:10.1089/jop.1996.12.409

PMID:8951677

Abstract

The effect of sodium hyaluronate (Na-HA) on the growth of rabbit corneal epithelial cells was investigated in vitro by using MTT assay and [3H]-thymidine incorporation. Na-HA, with a molecular weight of 86 x 10(4), enhanced the growth of the corneal epithelial cells cultured on a fibronectin (FN) coated dish, but when cells were cultured on a noncoated dish, Na-HA did not enhance the growth of the cells. Na-HA, labeled with 5-aminofluorescein (FA-HA), bound to the corneal epithelial cells, and the CD44-like molecule was identified on the cells by using a monoclonal antibody, OS/37. Furthermore, the binding of FA-HA to the cells was significantly inhibited by the monoclonal antibody. These results suggest that Na-HA binds to the CD44-like molecule associated with FN and then enhances the growth of the corneal epithelial cells.

摘要

采用MTT法和[3H]-胸腺嘧啶核苷掺入法，在体外研究了透明质酸钠（Na-HA）对兔角膜上皮细胞生长的影响。分子量为86×10(4)的Na-HA可促进在纤连蛋白（FN）包被培养皿上培养的角膜上皮细胞的生长，但当细胞在未包被的培养皿上培养时，Na-HA并不能促进细胞生长。用5-氨基荧光素标记的Na-HA（FA-HA）可与角膜上皮细胞结合，通过单克隆抗体OS/37在细胞上鉴定出类CD44分子。此外，单克隆抗体可显著抑制FA-HA与细胞的结合。这些结果表明，Na-HA与FN相关的类CD44分子结合，进而促进角膜上皮细胞的生长。