Asari A, Morita M, Sekiguchi T, Okamura K, Horie K, Miyauchi S
Tokyo Research Institute, Seikagaku Corporation, Japan.
Jpn J Ophthalmol. 1996;40(1):18-25.
Changes in hyaluronan (HA) concentration and stainability were investigated in the rabbit cornea after wounds made by exposure to n-heptanol. The HA concentration in the cornea increased gradually until day 14 after wounding, and then decreased. The HA concentration returned to the normal level 56 days after wounding. In the normal control cornea, HA staining was observed in the epithelium and stroma. The intensity of HA staining in the epithelium and stroma increased until day 3 after wounding, when the epithelium had completely covered the defect. At day 28, when the thickness of the corneal epithelium returned to the normal level, the intensity of HA staining in the epithelium also decreased. However, staining in the stroma was still strong. HA staining in the stroma decreased by day 56 after wounding. In parallel experiments, the immunostaining for CD44, an HA receptor, and fibronectin (FN) was carried out in the same model. The immunostaining in the epithelium of both CD44 and FN was synchronistic with the HA staining during the early stages after wounding. These events suggest that HA, CD44 and FN cooperatively play important roles in corneal epithelial wound healing.
研究了暴露于正庚醇造成创伤后兔角膜中透明质酸（HA）浓度和染色性的变化。角膜中的HA浓度在创伤后逐渐升高，直至第14天，然后下降。创伤后56天，HA浓度恢复至正常水平。在正常对照角膜中，上皮和基质中均观察到HA染色。上皮和基质中HA染色强度在创伤后第3天增加，此时上皮已完全覆盖缺损。在第28天，当角膜上皮厚度恢复至正常水平时，上皮中HA染色强度也下降。然而，基质中的染色仍然很强。创伤后第56天，基质中的HA染色下降。在平行实验中，在同一模型中对HA受体CD44和纤连蛋白（FN）进行了免疫染色。创伤后早期，上皮中CD44和FN的免疫染色与HA染色同步。这些结果表明，HA、CD44和FN在角膜上皮创伤愈合中协同发挥重要作用。
