使用正相高效液相色谱法和光电二极管阵列检测法分析和纯化佛波酯

Analysis and purification of phorbol esters using normal phase HPLC and photodiode-array detection.

作者信息

Dimitrijević S M, Humer U, Shehadeh M, Ryves W J, Hassan N M, Evans F J

机构信息

Department of Pharmacognosy, School of Pharmacy, University of London, UK.

出版信息

J Pharm Biomed Anal. 1996 Dec;15(3):393-401. doi: 10.1016/s0731-7085(96)01867-5.

DOI:10.1016/s0731-7085(96)01867-5

PMID:8951701

Abstract

For the first time a normal-phase HPLC method using photodiode-array detection is described for the analysis and purification of phorbol esters. The use of the method is demonstrated with examples of 10 different tigliane and daphnane esters (TPA, DOPP, DOPPA, Sap A, Sap B, Sap C, Sap D, Thy A, Ro and Rx). Both analytical and semipreparative techniques were developed. The method has been used in the final purification of DOPP and Rx from plant extracts. The method can be employed in the areas of phytochemistry, biochemistry and pharmacology/toxicology, where small samples of the toxic materials are required for research.

摘要

首次描述了一种使用光电二极管阵列检测的正相高效液相色谱法，用于佛波酯的分析和纯化。通过10种不同的大戟烷和瑞香烷酯（TPA、DOPP、DOPPA、Sap A、Sap B、Sap C、Sap D、Thy A、Ro和Rx）的示例展示了该方法的应用。开发了分析和半制备技术。该方法已用于从植物提取物中最终纯化DOPP和Rx。该方法可用于植物化学、生物化学和药理学/毒理学领域，这些领域的研究需要少量有毒物质样本。