Alexandre E, Graba Y, Fasano L, Gallet A, Perrin L, De Zulueta P, Pradel J, Kerridge S, Jacq B
Laboratoire de Génétique et Physiologie du Développement, IBDM, Marseille, France.
Mech Dev. 1996 Oct;59(2):191-204. doi: 10.1016/0925-4773(96)00594-1.
The Drosophila teashirt (tsh) gene has an homeotic function which, in combination with HOM-C genes, determines thoracic and abdominal (trunk) identities. Analysis of TSH protein distribution during embryogenesis using a specific polyclonal antibody shows that it is nuclear. The protein is present with regional modulation in several tissues within the trunk, suggesting additional tsh functions to those already studied. We identified a candidate tsh target shared with some HOM-C genes, the modifier of variegation gene modulo (mod). The TSH zinc-finger protein recognizes in vitro two specific sites within a 5' control element of the mod gene which responds in vivo to tsh activity. TSH is therefore a DNA binding protein and might directly control mod expression.
果蝇的teashirt（tsh）基因具有同源异型功能，它与同源异型复合体（HOM-C）基因共同作用，决定胸腹部（躯干）的特征。使用特异性多克隆抗体对胚胎发育过程中TSH蛋白分布进行分析，结果表明它定位于细胞核。该蛋白在躯干内的多个组织中呈现区域调节性分布，提示其具有一些尚未被研究的功能。我们鉴定出一个与某些HOM-C基因共有的tsh候选靶标，即杂色修饰基因模体（mod）。TSH锌指蛋白在体外识别mod基因5'调控元件内的两个特定位点，该调控元件在体内对tsh活性产生响应。因此，TSH是一种DNA结合蛋白，可能直接控制mod的表达。
