Prows D R, Murphy E J, Moncecchi D, Schroeder F
Division of Pharmacology and Medicinal Chemistry, University of Cincinnati, College of Pharmacy, OH 45267-0004, USA.
Chem Phys Lipids. 1996 Nov 1;84(1):47-56. doi: 10.1016/s0009-3084(96)02619-9.
The effect of intestinal fatty acid binding protein (I-FABP) expression on cell growth and cell lipid content is not known. Therefore, mouse L-cell fibroblasts were transfected with the cDNA encoding for I-FABP. The high expression clones expressed 0.35% of the total cytosolic proteins as I-FABP. Mock transfected L-cells did not differ from control L-cells in any properties tested. Neither the growth rate, maximal cell density, nor [3H]oleic acid uptake differed in I-FABP expressing as compared to control cells. In contrast, I-FABP expression increased triacylglycerol and cholesteryl ester mass (nmol/mg protein) by 63% and 25%, respectively. Phospholipid mass was unchanged in I-FABP expressing cells. The initial [3H]oleic acid esterification into triacylglycerols and cholesteryl esters was increased 3.9- and 2.5-fold in I-FABP expressing cells. Although, the initial [3H]oleic acid esterification into total phospholipids was unchanged, within the phospholipid fraction the initial [3H]oleic acid esterification into phosphatidylethanolamine was increased 70% and decreased 50% in phosphatidylcholine in I-FABP expressing cells. These observed differences suggest a distinct role for I-FABP in stimulating net formation, and not just turnover, of triacylglycerides and cholesteryl esters in transfected L-cell fibroblasts.
肠道脂肪酸结合蛋白（I-FABP）表达对细胞生长和细胞脂质含量的影响尚不清楚。因此，用编码I-FABP的cDNA转染小鼠L细胞成纤维细胞。高表达克隆表达的I-FABP占总胞质蛋白的0.35%。模拟转染的L细胞在任何测试特性上与对照L细胞均无差异。与对照细胞相比，I-FABP表达细胞的生长速率、最大细胞密度以及[3H]油酸摄取均无差异。相反，I-FABP表达分别使三酰甘油和胆固醇酯质量（nmol/mg蛋白）增加了63%和25%。I-FABP表达细胞中的磷脂质量未发生变化。I-FABP表达细胞中[3H]油酸最初酯化生成三酰甘油和胆固醇酯的过程分别增加了3.9倍和2.5倍。虽然[3H]油酸最初酯化生成总磷脂的过程未发生变化，但在磷脂组分中，I-FABP表达细胞中[3H]油酸最初酯化生成磷脂酰乙醇胺的过程增加了70%，而生成磷脂酰胆碱的过程减少了50%。这些观察到的差异表明，I-FABP在刺激转染的L细胞成纤维细胞中三酰甘油和胆固醇酯的净合成而非仅仅周转方面具有独特作用。
