Razin S, Pfendt E A, Matsumura T, Hayflick L
Mech Ageing Dev. 1977 Sep-Oct;6(5):379-84. doi: 10.1016/0047-6374(77)90039-2.
The biosynthetic abilities of WI-38 fibroblasts from early and late population-doubling-level cultures were compared by autoradiography of cells grown with labeled precursors of DNA, RNA, protein and lipids. Incorporation of radioactive thymidine, uridine, protein-hydrolysate, acetate, oleic acid and cholesterol, as measured by the number of grains per cell surface, decreased with the progressive aging of the culture. However, the decrease in the incorporation of acetate, oleic acid and cholesterol was much smaller than that of the other precursors, indicating that lipid synthesis is affected to a lesser degree than protein and nucleic acid synthesis on aging. This result is in accord with the higher lipid content and proliferation of intracellular membranes in cells of "old" WI-38 cultures reported by others.
通过对用DNA、RNA、蛋白质和脂质的标记前体培养的细胞进行放射自显影，比较了来自早期和晚期群体倍增水平培养物的WI-38成纤维细胞的生物合成能力。以每细胞表面的颗粒数衡量，放射性胸苷、尿苷、蛋白质水解物、乙酸盐、油酸和胆固醇的掺入量随着培养物的逐渐老化而降低。然而，乙酸盐、油酸和胆固醇掺入量的降低比其他前体小得多，表明在老化过程中脂质合成受到的影响程度小于蛋白质和核酸合成。这一结果与其他人报道的“老”WI-38培养物细胞中较高的脂质含量和细胞内膜的增殖一致。
