D'Arena G, Musto P, Cascavilla N, Di Giorgio G, Zendoli F, Carotenuto M
Division of Hematology, IRCCS Casa Sollievo della Sofferenza Hospital, San Giovanni Rotondo, Italy.
Haematologica. 1996 Sep-Oct;81(5):404-9.
Human umbilical cord blood (HUCB) is a possible alternative to bone marrow (BM) and mobilized peripheral blood (PB) for transplantation of hematopoietic progenitors. The aim of this study was to evaluate the phenotypic profile of CD34+ progenitors present in HUCB.
A flow cytometric analysis was performed on 20 HUCB samples, using a large panel of monoclonal antibodies recognizing different lineage or activation antigens, in double labeling with CD34.
A toal of 13,897 +/- 2,529 cells/microL, 0.84 +/- 0.83% of which were CD34+, was found. The large majority of CD34+ cells were committed toward initial myeloid differentiation (CD33+, CD13+) and expressed the transferrin receptor (CD71). A substantial proportion of these cells (about 40%) co-expressed CD45RA and CD117, while a very small number displayed markers of advanced myeloid commitment, such as CD14, CD15 and CD41 (less than 2%), or those of lymphoid differentiation: CD2, CD5, CD7, CD10 and CD19 (less than 6%). About 11% of HUCB CD34+ cells were primitive progenitors, as suggested by the absence of HLA-DR and CD38 on their surface.
As previously observed in BM and mobilized PB, the phenotype of HUCB CD34+ cells is quite heterogeneous. In particular, HUCB contains subpopulations of both early and committed hematopoietic progenitors which may represent a valid source for transplantation.
人脐带血（HUCB）可能是骨髓（BM）和动员外周血（PB）用于造血祖细胞移植的替代物。本研究的目的是评估HUCB中存在的CD34+祖细胞的表型特征。
使用一大组识别不同谱系或活化抗原的单克隆抗体，对20份HUCB样本进行流式细胞术分析，并与CD34进行双标记。
发现每微升中有13,897±2,529个细胞，其中0.84±0.83%为CD34+细胞。绝大多数CD34+细胞倾向于初始髓系分化（CD33+、CD13+）并表达转铁蛋白受体（CD71）。这些细胞中有相当一部分（约40%）共表达CD45RA和CD117，而极少数细胞显示出晚期髓系分化的标志物，如CD14、CD15和CD41（少于2%），或淋巴系分化的标志物：CD2、CD5、CD7、CD10和CD19（少于6%）。约11%的HUCB CD34+细胞为原始祖细胞，这可由其表面缺乏HLA-DR和CD38表明。
如先前在BM和动员PB中所观察到的，HUCB CD34+细胞的表型相当异质性。特别是，HUCB包含早期和定向造血祖细胞亚群，这可能是移植的有效来源。
