de Sá Silva Fernando, Almeida Paula Nascimento, Rettore João Vitor Paes, Maranduba Claudinéia Pereira, de Souza Camila Maurmann, de Souza Gustavo Torres, Zanette Rafaella de Souza Salomão, Miyagi Sueli Patricia Harumi, Santos Marcelo de Oliveira, Marques Márcia Martins, Maranduba Carlos Magno da Costa
Post-Graduation Program in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
J Biomed Biotechnol. 2012;2012:758102. doi: 10.1155/2012/758102. Epub 2012 Nov 20.
Stem cells, both embryonic and adult, due to the potential for application in tissue regeneration have been the target of interest to the world scientific community. In fact, stem cells can be considered revolutionary in the field of medicine, especially in the treatment of a wide range of human diseases. However, caution is needed in the clinical application of such cells and this is an issue that demands more studies. This paper will discuss some controversial issues of importance for achieving cell therapy safety and success. Particularly, the following aspects of stem cell biology will be presented: methods for stem cells culture, teratogenic or tumorigenic potential, cellular dose, proliferation, senescence, karyotyping, and immunosuppressive activity.
胚胎干细胞和成体干细胞由于在组织再生中的应用潜力，一直是世界科学界关注的焦点。事实上，干细胞在医学领域可被视为具有革命性，尤其是在治疗多种人类疾病方面。然而，此类细胞的临床应用需要谨慎，这是一个需要更多研究的问题。本文将讨论一些对于实现细胞治疗的安全性和成功至关重要的争议性问题。特别是，将介绍干细胞生物学的以下几个方面：干细胞培养方法、致畸或致瘤潜力、细胞剂量、增殖、衰老、核型分析以及免疫抑制活性。
