Sato K, Tomioka H, Saito H, Kawahara S
Department of Microbiology and Immunology, Shimane Medical University, Japan.
Kansenshogaku Zasshi. 1996 Oct;70(10):1103-10. doi: 10.11150/kansenshogakuzasshi1970.70.1103.
A newly devised 7H12 cultivation method combined with nested PCR assay using Mycobacterium tuberculosis-specific primers was evaluated for its usefulness to detect and identify tubercle bacilli in 38 sputum samples. This method yielded detection and identification of M. tuberculosis in sputum specimens with excellent sensitivity, specificity, and rapidity, when compared to the cultivation method using Ogawa egg medium or the 7H12 cultivation method in which bacterial growth is monitored by microscopy (7H12-microscopy method) with the same sensitivity as that of the BACTEC 460 TB system.
一种新设计的7H12培养方法结合使用结核分枝杆菌特异性引物的巢式PCR检测法，在38份痰液样本中评估了其检测和鉴定结核杆菌的效用。与使用小川卵培养基的培养方法或通过显微镜监测细菌生长的7H12培养方法（7H12显微镜法）相比，该方法在痰液标本中检测和鉴定结核分枝杆菌时具有出色的敏感性、特异性和快速性，其敏感性与BACTEC 460 TB系统相同。
