评价一种快速检测方法，用于鉴定固体和液体培养基中生长的结核分枝杆菌。
Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media.
机构信息
Infectious Disease Section, Department of Medicine, University of the Philippines-Philippine General Hospital, Manila, Philippines.
出版信息
Int J Tuberc Lung Dis. 2011 Nov;15(11):1475-7, i. doi: 10.5588/ijtld.10.0709.
An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation of M. tuberculosis in acidfast bacilli positive cultures.
一种 MPT64 抗原检测试剂盒，即 SD Bioline TB Ag MPT64 Rapid 试剂盒，用于鉴定在固体和液体培养基中生长的结核分枝杆菌。该快速检测法在奥格瓦（Ogawa）培养基上生长的 133 株结核分枝杆菌培养物中的 132 株（99.2%）和液体培养基中生长的 143 株培养物中的 136 株（95.1%）中呈阳性结果，总灵敏度为 97.1%。所有 18 株非结核分枝杆菌的快速检测均为阴性，特异性为 100%。该快速检测法似乎可用于初始确认抗酸杆菌阳性培养物中的结核分枝杆菌。
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