Simon O R, Powell N, Davis P, Gossell M
Department of Pharmacology, University of the West Indies, Jamaica.
West Indian Med J. 1996 Sep;45(3):89-91.
Electrical field stimulation of guinea-pig tracheal muscle strips produced a frequency-dependent biphasic response consisting of an initial cholinergic contraction followed by relaxation. Both phases of the response were of neural origin. In the presence of methylene blue, a guanylate cyclase inhibitor, the resting tension and the contraction were increased, but the accompanying relaxation was inhibited. However, in the presence of sodium nitroprusside, a guanylate cyclase activator, the resting tension was reduced and the contraction was inhibited, but the relaxation was prolonged and increased. Similarly, in the presence of either 3-isobutyl-1-methylxanthine, which promotes cyclic guanosine monophosphate (cGMP) accumulation, or 8-bromo-cGMP, an analogue of cGMP, the resting tension was reduced and the contraction was inhibited but the relaxation was prolonged and increased. From these results, it is concluded that guanylate cyclase is involved in modulating the resting tension and the neurally-induced contraction of guinea-pig tracheal muscle.
对豚鼠气管肌条进行电场刺激可产生频率依赖性双相反应，该反应由最初的胆碱能收缩继以舒张组成。反应的两个阶段均起源于神经。在存在鸟苷酸环化酶抑制剂亚甲蓝的情况下，静息张力和收缩增强，但随之而来的舒张受到抑制。然而，在存在鸟苷酸环化酶激活剂硝普钠的情况下，静息张力降低，收缩受到抑制，但舒张延长且增强。同样，在存在促进环磷酸鸟苷（cGMP）积累的3-异丁基-1-甲基黄嘌呤或cGMP类似物8-溴-cGMP的情况下，静息张力降低，收缩受到抑制，但舒张延长且增强。从这些结果可以得出结论，鸟苷酸环化酶参与调节豚鼠气管肌的静息张力和神经诱导的收缩。
