Marques M J, Santo Neto H, Meirelles U M
Department of Anatomy, Faculty of Medical Sciences, Campinas State University, SP, Brazil.
Acta Physiol Pharmacol Ther Latinoam. 1998;48(2):83-7.
The aim of the present investigation was to evaluate the potential modulatory effect of the guinea pig tracheal smooth muscle intrinsic tone on the relaxant responses to sodium nitroprusside and the effects of methylene blue on this response. Paired tracheal chains were mounted for isotonic contractions under 500 mg of tension in Krebs-Henseleit solution. The intrinsic modulatory tone was inhibited by indomethacin, in a concentration that did not have any effect over carbachol induced contractions. Sodium nitroprusside-induced relaxations were the same in the absence or presence of the modulatory tone. Methylene blue inhibited 50% sodium nitroprusside-induced relaxations, in the presence or absence of the intrinsic system. This suggests that sodium nitroprusside-induced relaxations are mediated through guanylyl-cyclase activation and that these are not under the modulation of the intrinsic prostaglandinergic tone.
本研究的目的是评估豚鼠气管平滑肌固有张力对硝普钠舒张反应的潜在调节作用，以及亚甲蓝对该反应的影响。将成对的气管链安装在Krebs-Henseleit溶液中，在500mg张力下进行等张收缩。吲哚美辛可抑制固有调节张力，其浓度对卡巴胆碱诱导的收缩无任何影响。在存在或不存在调节张力的情况下，硝普钠诱导的舒张作用相同。在存在或不存在固有系统的情况下，亚甲蓝均可抑制50%的硝普钠诱导的舒张作用。这表明硝普钠诱导的舒张作用是通过鸟苷酸环化酶激活介导的，且不受固有前列腺素能张力的调节。
