Sipes S L, Medaglia M V, Stabley D L, DeBruyn C S, Alden M S, Catenacci V, Landel C P
Department of Clinical Science, Nemours Research Programs, Alfred I. duPont Institute, PO Box 269, Wilmington, DE 19899, USA.
Immunogenetics. 1996;45(2):108-20. doi: 10.1007/s002510050178.
Because of the role major histocompatibility complex (MHC) class I b molecules may play during mouse embryonic development, we thought it would be interesting to search for additional MHC class I b molecules that might be expressed in preimplantation embryos, and in particular in the trophoblastic lineage. We therefore screened a mouse preimplantation blastocyst cDNA library for MHC class I sequences. This search led to the identification and characterization of a new MHC class I b gene, blastocyst MHC. Sequences identical to the exons and 3' untranslated region of this gene have been found in many laboratory mouse strains, as well as in the related mouse species Mus spreciligus. The presence of this gene in mouse strains of different MHC class I haplotypes argues that blastocyst MHC is a unique, newly-described gene rather than a new allele of a previously described mouse MHC class I gene. Blastocyst MHC has the structure of an MHC class I b gene, with the six exons characteristic of T-region genes. It is linked to H2-D. The amino acid sequence encoded by this gene maintains all the features of a functional antigen-presentation domain. The blastocyst MHC gene, like the human class I b gene HLA-G, is expressed at the blastocyst stage and in the placenta, and may be the mouse analog for HLA-G.
由于主要组织相容性复合体（MHC）I类b分子在小鼠胚胎发育过程中可能发挥的作用，我们认为寻找可能在植入前胚胎中，特别是在滋养层谱系中表达的其他MHC I类b分子会很有意思。因此，我们筛选了小鼠植入前囊胚cDNA文库以寻找MHC I类序列。这项搜索导致了一个新的MHC I类b基因——囊胚MHC的鉴定和表征。在许多实验室小鼠品系以及相关小鼠物种小家鼠中都发现了与该基因的外显子和3'非翻译区相同的序列。该基因在不同MHC I类单倍型的小鼠品系中的存在表明，囊胚MHC是一个独特的、新描述的基因，而不是先前描述的小鼠MHC I类基因的新等位基因。囊胚MHC具有MHC I类b基因的结构，具有T区域基因特有的六个外显子。它与H2-D连锁。该基因编码的氨基酸序列保留了功能性抗原呈递结构域的所有特征。囊胚MHC基因与人类I类b基因HLA-G一样，在囊胚阶段和胎盘中表达，可能是HLA-G的小鼠类似物。
