Landel C P, Stabley D L, Bundesen L Q
Department of Clinical Science, Alfred I. duPont Institute, Wilmington, DE 19899, USA.
J Reprod Immunol. 1997 Apr;33(1):31-43. doi: 10.1016/s0165-0378(96)01013-3.
We used an RT-PCR based strategy to amplify, clone and sequence MHC class I genes transcribed in the blastocyst and placenta of BALB/c mice. The PCR primers used were capable of amplifying many novel class I sequences from genomic DNA. By comparing the resulting sequence data with known class I sequences, we identified a number of different class I genes transcribed in these tissues. These include H2-K, -D, -L and a novel sequence in blastocysts, and H2-K, -D, -L, -D2, -T9, -T13, -T17, -T18, -M2 and three additional novel sequences in placenta. We postulate that some members of this spectrum of blastocyst and placentally-expressed MHC class Ib genes may act together at the maternal-fetal interface in ways that are important for a successful pregnancy.
我们采用基于逆转录聚合酶链反应（RT-PCR）的策略，对BALB/c小鼠囊胚和胎盘中转录的主要组织相容性复合体（MHC）I类基因进行扩增、克隆及测序。所使用的PCR引物能够从基因组DNA中扩增出许多新的I类序列。通过将所得序列数据与已知的I类序列进行比较，我们在这些组织中鉴定出了一些不同的I类基因。这些基因包括囊胚中的H2-K、-D、-L和一个新序列，以及胎盘中的H2-K、-D、-L、-D2、-T9、-T13、-T17、-T18、-M2和另外三个新序列。我们推测，囊胚和胎盘表达的这一系列MHC Ib类基因中的一些成员可能在母胎界面共同发挥作用，对成功妊娠具有重要意义。
