PCR identification of class I major histocompatibility complex genes transcribed in mouse blastocyst and placenta.

We used an RT-PCR based strategy to amplify, clone and sequence MHC class I genes transcribed in the blastocyst and placenta of BALB/c mice. The PCR primers used were capable of amplifying many novel class I sequences from genomic DNA. By comparing the resulting sequence data with known class I sequences, we identified a number of different class I genes transcribed in these tissues. These include H2-K, -D, -L and a novel sequence in blastocysts, and H2-K, -D, -L, -D2, -T9, -T13, -T17, -T18, -M2 and three additional novel sequences in placenta. We postulate that some members of this spectrum of blastocyst and placentally-expressed MHC class Ib genes may act together at the maternal-fetal interface in ways that are important for a successful pregnancy.