Stockert J C, Bertolesi G E
Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Spain.
Micron. 1996 Jun-Aug;27(3-4):177-9. doi: 10.1016/0968-4328(96)00032-7.
Peritoneal mast cells and lymphocytes from mice were placed on graphite supports, fixed in methanol, stained with the new fluorochrome tris(2,2'-bipyridine)ruthenium(II) and microanalysed using scanning electron microscopy (SEM). X-ray microanalysis showed the expected signal of P and S (K alpha lines) in emission spectra of lymphocytes and mast cells. The signal of Ru (L alpha 1 and L beta 1) overlapped with that of Cl, although the peak in the corresponding region was about 7 times higher than that from unstained cells. X-ray images showed the topographic localization of P, S and Ru in mast cells and lymphocytes and confirmed the accumulation of the Ru complex in heparin- and DNA-containing structures. These results indicate that, by using suitable marker elements and detection methods, analytical SEM is a useful complement in cytochemical studies.
将小鼠的腹膜肥大细胞和淋巴细胞置于石墨载体上，用甲醇固定，用新型荧光染料三（2,2'-联吡啶）钌（II）染色，然后使用扫描电子显微镜（SEM）进行微分析。X射线微分析显示淋巴细胞和肥大细胞发射光谱中预期的P和S信号（Kα线）。Ru（Lα1和Lβ1）的信号与Cl的信号重叠，尽管相应区域的峰值比未染色细胞的峰值高约7倍。X射线图像显示了肥大细胞和淋巴细胞中P、S和Ru的地形定位，并证实了Ru复合物在含肝素和DNA结构中的积累。这些结果表明，通过使用合适的标记元素和检测方法，分析扫描电子显微镜在细胞化学研究中是一种有用的补充。
