Freitas dos Santos L M, Leak D J, Livingston A G
Department of Chemical Engineering and Chemical Technology, Imperial College, London, United Kingdom.
Appl Environ Microbiol. 1996 Dec;62(12):4675-7. doi: 10.1128/aem.62.12.4675-4677.1996.
1,2-dibromoethane (DBE) is a common environmental contaminant; it is potentially carcinogenic and has been detected in soil and groundwater supplies. Most of the biodegradation studies to date have been performed under anaerobic conditions or in the context of soil remediation, where the pollutant concentration was in the parts per billion range. In this work a mixed bacterial culture capable of complete aerobic mineralization of concentrations of DBE up to 1 g liter(-1) under well-controlled laboratory conditions was enriched. In order to verify biodegradation, formation of biodegradation products as well as the disappearance of DBE from the biological medium were measured. Complete mineralization was verified by measuring stoichiometric release of the biodegradation products. This mixed culture was found to be capable of degrading other halogenated compounds, including bromoethanol, the degradation of which has not been reported previously.
1,2 - 二溴乙烷(DBE)是一种常见的环境污染物;它具有潜在致癌性,已在土壤和地下水源中被检测到。迄今为止,大多数生物降解研究都是在厌氧条件下或土壤修复的背景下进行的,其中污染物浓度处于十亿分之一的范围内。在这项工作中,富集了一种混合细菌培养物,该培养物在严格控制的实验室条件下能够对浓度高达1克/升的DBE进行完全好氧矿化。为了验证生物降解,测量了生物降解产物的形成以及DBE从生物介质中的消失情况。通过测量生物降解产物的化学计量释放来验证完全矿化。发现这种混合培养物能够降解其他卤代化合物,包括溴乙醇,其降解情况此前尚未见报道。