Zange J, Müller K, Gerzer R, Sippel K, Wehling M
Institute of Clinical Pharmacology, Faculty for Clinical Medicine Mannheim, University of Heidelberg, Mannheim, Germany.
J Clin Endocrinol Metab. 1996 Dec;81(12):4296-300. doi: 10.1210/jcem.81.12.8954030.
Nongenomic in vitro effects of aldosterone on the sodium-proton antiport and intracellular second messengers have been described in human mononuclear leukocytes, vascular smooth muscle cells, and endothelial cells. To test the potential physiological relevance of these effects, an in vivo 31P magnetic resonance spectroscopy study on the human calf at rest and during exercise was performed in 10 healthy volunteers receiving either 1 mg aldosterone or placebo iv in a double blind, randomized, cross-over trial. Spectra were analyzed for phosphocreatine, ATP, phosphomonoesters, inorganic intracellular phosphate, and intracellular pH. Resting values remained unchanged by aldosterone. After isometric contraction of the calf (50% body weight for 3 min), phosphocreatine recovered to significantly higher levels after application of aldosterone compared with placebo. Other parameters were not significantly changed by aldosterone. Effects appeared immediately after isometric contraction and, thus, occurred within 8 min of aldosterone administration. They are, therefore, likely to represent the first contemporary evidence of nongenomic in vivo effects of aldosterone in man. These findings also point to an involvement of aldosteron in the acute stress adaptation of cellular oxidative metabolism in human muscle physiology.
醛固酮对人单核白细胞、血管平滑肌细胞和内皮细胞的钠-质子逆向转运及细胞内第二信使的非基因组体外效应已有描述。为了检验这些效应的潜在生理相关性，在一项双盲、随机、交叉试验中，对10名健康志愿者进行了一项体内31P磁共振波谱研究，观察他们在静息和运动状态下小腿的情况，这些志愿者静脉注射1mg醛固酮或安慰剂。分析了磷酸肌酸、三磷酸腺苷（ATP）、磷酸单酯、细胞内无机磷酸盐和细胞内pH值的波谱。醛固酮对静息值无影响。小腿等长收缩（相当于体重的50%，持续3分钟）后，与安慰剂相比，应用醛固酮后磷酸肌酸恢复到显著更高的水平。醛固酮对其他参数无显著影响。等长收缩后效应立即出现，即在醛固酮给药后8分钟内出现。因此，它们可能是醛固酮在人体非基因组体内效应的首个当代证据。这些发现还表明醛固酮参与了人体肌肉生理学中细胞氧化代谢的急性应激适应过程。
