编码甜味蛋白奇异果甜蛋白的合成基因在大肠杆菌中的表达。
Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli.
作者信息
Faus I, Patiño C, Río J L, del Moral C, Barroso H S, Rubio V
机构信息
Laboratory of Biotechnology, URQUIMA, S.A. (URIACH GROUP), Sant Fost de Campsenteiles (Barcelona), Spain.
出版信息
Biochem Biophys Res Commun. 1996 Dec 4;229(1):121-7. doi: 10.1006/bbrc.1996.1767.
PMID:8954093
Abstract
A synthetic gene encoding the aminoacid sequence of the sweet-tasting protein thaumatin II has been assembled and expressed in Escherichia coli. Immunoblotting analysis shows that the expressed recombinant thaumatin has the same molecular weight as the protein from its natural source, the plant Thaumatoccocus daniellii Benth.
摘要
一个编码甜味蛋白奇异果甜蛋白II氨基酸序列的合成基因已被组装并在大肠杆菌中表达。免疫印迹分析表明，所表达的重组奇异果甜蛋白与来自其天然来源——植物丹尼氏奇异果（Thaumatoccocus daniellii Benth.）的蛋白具有相同的分子量。
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