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基于快速高效液相色谱的高碘酸盐偶联硫代巴比妥酸法测定支气管肺泡灌洗液中的唾液酸

Rapid, high performance liquid chromatography-based, periodate-coupled thiobarbituric acid method for the determination of sialic acid in bronchoalveolar lavage fluid.

作者信息

Freyberger A

机构信息

Bayer AG, Fachbereich Toxikologie, Wuppertal, Germany.

出版信息

Exp Toxicol Pathol. 1996 Nov;48(6):526-8. doi: 10.1016/S0940-2993(96)80074-1.

DOI:10.1016/S0940-2993(96)80074-1
PMID:8954340
Abstract

A simple, rapid high performance liquid chromatographic adaptation of the periodate-coupled thiobarbituric acid (TBA) method was developed for the quantification of sialic acid (SIAC) in supernatants from bronchoalveolar lavage fluids (BALF). Malondialdehyde released from BALF samples in various amounts did not interfere with the accurate quantification of SIAC.

摘要

开发了一种简单、快速的高效液相色谱法,用于对支气管肺泡灌洗(BALF)上清液中的唾液酸(SIAC)进行定量,该方法是对高碘酸盐偶联硫代巴比妥酸(TBA)法的改进。BALF样本中释放的不同量的丙二醛不会干扰SIAC的准确定量。

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