Gutteridge J M, Mumby S, Koizumi M, Taniguchi N
Oxygen Chemistry Laboratory, Unit of Critical Care, Department of AICU, Royal Brompton Hospital, Imperial College of Science Technology and Medicine, Sydney Street, London, SW3 6NP, United Kingdom.
Biochem Biophys Res Commun. 1996 Dec 24;229(3):806-9. doi: 10.1006/bbrc.1996.1884.
Plasma from certain preterm and term babies has recently been shown to contain low molecular mass iron (LMrFe) that can be chelated and measured in the bleomycin assay. The chemical nature of such iron, when detected in biological fluids, is still unclear with suggestions that it may not be a redox active form of iron until it becomes bound to bleomycin. Here we show that all plasma samples, from umbilical cord bloods, containing bleomycin-detectable iron, activated the enzyme aconitase in a cell-free system. It is, therefore, highly likely that LMrFe detected in the bleomycin assay is a biologically redox active form of iron.
最近有研究表明，某些早产儿和足月儿的血浆中含有低分子量铁（LMrFe），这种铁可以在博来霉素检测法中被螯合并测定。当在生物体液中检测到这种铁时，其化学性质仍不清楚，有人认为它可能不是铁的氧化还原活性形式，直到它与博来霉素结合。在这里，我们表明，所有来自脐带血且含有可被博来霉素检测到的铁的血浆样本，在无细胞系统中都能激活乌头酸酶。因此，在博来霉素检测法中检测到的LMrFe极有可能是铁的一种具有生物氧化还原活性的形式。
