酵母双杂交系统检测枯草芽孢杆菌σB调节因子之间的相互作用。
The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
作者信息
Voelker U, Voelker A, Haldenwang W G
机构信息
Institute für Mikrobiologie und Molecularbiologié, Einst-Moritz-Arndt-Universität, Greifswald, Germany.
出版信息
J Bacteriol. 1996 Dec;178(23):7020-3. doi: 10.1128/jb.178.23.7020-7023.1996.
Abstract
SigmaB, the general stress response sigma factor of Bacillus subtilis, is regulated by the products of seven genes (rsbR, S, T, U, V, W, and X) with which it is cotranscribed. Biochemical techniques previously revealed physical associations among RsbW, RsbV, and sigmaB but failed to detect interactions of RsbR, S, T, U, or X with each other or RsbV, RsbW, or sigmaB. Using the yeast two-hybrid system, we have now obtained evidence for such interactions. The yeast reporter system was activated when RsbS was paired with either RsbR or RsbT, RsbR was paired with RsbT, and RsbV was paired with either RsbU or RsbW. In addition, RsbW2 and RsbR2 dimer formation was detected. RsbX failed to show interactions with itself or any of the other sigB operon products.
摘要
SigmaB是枯草芽孢杆菌的一般应激反应σ因子，由与其共转录的7个基因（rsbR、S、T、U、V、W和X）的产物调控。先前的生化技术揭示了RsbW、RsbV和sigmaB之间的物理关联，但未能检测到RsbR、S、T、U或X彼此之间以及与RsbV、RsbW或sigmaB的相互作用。利用酵母双杂交系统，我们现在获得了此类相互作用的证据。当RsbS与RsbR或RsbT配对、RsbR与RsbT配对以及RsbV与RsbU或RsbW配对时，酵母报告系统被激活。此外，还检测到了RsbW2和RsbR2二聚体的形成。RsbX未能显示出与自身或sigB操纵子的任何其他产物的相互作用。
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本文引用的文献
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Reactivation of the Bacillus subtilis anti-sigma B antagonist, RsbV, by stress- or starvation-induced phosphatase activities.枯草芽孢杆菌抗σB拮抗剂RsbV通过应激或饥饿诱导的磷酸酶活性重新激活
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