Takeuchi K, Ishihara M, Kawaura C, Noji M, Furuno T, Nakanishi M
Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Japan.
FEBS Lett. 1996 Nov 18;397(2-3):207-9. doi: 10.1016/s0014-5793(96)01136-2.
Cationic liposomes are known to be useful tools for gene transfection. However, the relation between transfection efficiency and physicochemical properties of liposomes has not been well understood. Here, we synthesized eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm. Transfection of plasmid pSV2CAT DNA into cells was done by cationic liposomes made of a mixture of dioleoylphosphatidylethanolamine (DOPE) and each cationic cholesterol derivative. At the same time we measured zeta potential of cationic liposomes by laser Doppler spectroscopy. The present results indicated that zeta potentials of cationic liposomes were well related to transfection activity of pSV2CAT DNA. This suggested that zeta potential of cationic liposomes is one of important factors which control gene transfection.
阳离子脂质体是已知的用于基因转染的有用工具。然而，脂质体的转染效率与其物理化学性质之间的关系尚未得到很好的理解。在此，我们合成了八种胆固醇阳离子衍生物，它们含有带有不同间隔臂的叔胺头部基团。通过由二油酰磷脂酰乙醇胺（DOPE）和每种阳离子胆固醇衍生物的混合物制成的阳离子脂质体将质粒pSV2CAT DNA转染到细胞中。同时，我们通过激光多普勒光谱法测量了阳离子脂质体的zeta电位。目前的结果表明，阳离子脂质体的zeta电位与pSV2CAT DNA的转染活性密切相关。这表明阳离子脂质体的zeta电位是控制基因转染的重要因素之一。
