通过操纵参与阿霉素（阿霉素）生物合成的天蓝色链霉菌dnrH和dnmT基因提高抗生素产量。
Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis.
作者信息
Scotti C, Hutchinson C R
机构信息
School of Pharmacy, University of Wisconsin, Madison 53706, USA.
出版信息
J Bacteriol. 1996 Dec;178(24):7316-21. doi: 10.1128/jb.178.24.7316-7321.1996.
Abstract
Sequence analysis of a 3.4-kb region Streptomyces peucetius daunorubicin (DNR) gene cluster established the presence of the dnrH and dnmT genes. In dnrH mutants, DNR production increased 8.5-fold, compared with that in the wild-type strain, while dnmT mutants accumulated epsilon-rhodomycinone (RHO), which normally becomes glycosylated in daunorubicin biosynthesis. Hence, dnmT may be involved in the biosynthesis or attachment of daunosamine to RHO or in the regulation of this process. Since the DnrH protein is similar to known glycosyl transferases, this protein may catalyze the conversion of DNR to its polyglycosylated forms, known as baumycins. Overexpression of dnmT in the wild-type and dnrH mutant strains resulted in a major decrease in RHO accumulation and increase in DNR production.
摘要
对3.4 kb的天蓝色链霉菌柔红霉素（DNR）基因簇进行序列分析，确定了dnrH和dnmT基因的存在。在dnrH突变体中，与野生型菌株相比，DNR产量增加了8.5倍，而dnmT突变体积累了ε-红霉酮（RHO），RHO在柔红霉素生物合成过程中通常会发生糖基化。因此，dnmT可能参与了柔红糖胺的生物合成或与RHO的连接，或参与了该过程的调控。由于DnrH蛋白与已知的糖基转移酶相似，该蛋白可能催化DNR转化为其多聚糖基化形式，即鲍曼霉素。在野生型和dnrH突变体菌株中过表达dnmT导致RHO积累大幅减少，DNR产量增加。
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