Ohashi Y, Minegishi M, Fujie H, Tsuchiya S, Ichinohazama R, Konno T
Department of Pediatric Oncology, Tohoku University, Sendai, Japan.
Acta Paediatr. 1996 Nov;85(11):1376-9. doi: 10.1111/j.1651-2227.1996.tb13931.x.
A 10-year-old patient with nasopharyngeal carcinoma (NPC) was studied for mutations within the carboxy-terminal portion of the Epstein-Barr virus (EBV) latent membrane protein (LMP)-1 gene. The EBV genome, defined as type A, was detected in biopsied tumor specimens by Southern hybridization with specific probes. Sequence analysis of the carboxy-terminal part of the LMP-1 gene revealed no deletions but seven single-base substitutions, four of which were found to be identical to those previously detected in codons for amino acids 322 to 366 in the Chinese NPC CAO. Although yet unresolved, the observed mutations may be associated with the pathogenesis of NPC.
对一名10岁的鼻咽癌（NPC）患者的爱泼斯坦-巴尔病毒（EBV）潜伏膜蛋白（LMP）-1基因羧基末端部分的突变情况进行了研究。通过用特异性探针进行Southern杂交，在活检的肿瘤标本中检测到定义为A型的EBV基因组。LMP-1基因羧基末端部分的序列分析显示没有缺失，但有七个单碱基替换，其中四个与先前在中国NPC CAO中第322至366位氨基酸密码子中检测到的相同。尽管尚未解决，但观察到的突变可能与NPC的发病机制有关。
