脂多糖诱导早孕滋养层细胞系中粒细胞集落刺激因子（G-CSF）和调节激活正常T细胞表达和分泌因子（RANTES）并使其进行翻译后表达
Induction and postranslational expression of G-CSF and RANTES in a first trimester trophoblast cell line by lipopolysaccharide.
作者信息
Svinarich D M, Bitonti O M, Araneda H, Romero R, Gonik B
机构信息
Department of Obstetrics and Gynecology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
出版信息
Am J Reprod Immunol. 1996 Nov;36(5):256-9. doi: 10.1111/j.1600-0897.1996.tb00173.x.
PROBLEM
Comparatively little is known about the capacity of first trimester trophoblasts to respond to an infection and coordinate an immune response. This study characterizes the LPS induction of G-CSF and RANTES in a first trimester trophoblast cell line.
METHODS
HTR-8/SV neo cells were exposed to LPS (1 micrograms/ml) for 0, 2, 4, 6, 8, and 24 hours in DMEM-Ham's F-12 media supplemented with 10% fetal bovine serum. Cytokine levels in culture supernatants were measured by ELISA. Northern analysis of total RNA was conducted using antisense cytokine probes.
RESULTS
Levels of immunoreactive G-CSF and RANTES from LPS induced cultures at 24 hours were 10-fold and 8.5-fold greater than cytokine levels from non-induced cells at 24 hours, respectively (P < 0.01). Under LPS induction, maximal rates of G-CSF and RANTES transcription occurred at 24 hours and 8 hours, respectively.
CONCLUSION
The LPS induction of proinflammatory cytokines in a first trimester trophoblast cell line supports the contention that first trimester trophoblasts participate in cytokine based immune signaling in response to infection.
问题
关于孕早期滋养层细胞对感染作出反应并协调免疫反应的能力，我们所知相对较少。本研究对孕早期滋养层细胞系中脂多糖（LPS）诱导粒细胞集落刺激因子（G-CSF）和调节激活正常T细胞表达和分泌因子（RANTES）的情况进行了表征。
方法
将HTR-8/SV neo细胞在补充有10%胎牛血清的DMEM-Ham's F-12培养基中，分别暴露于脂多糖（1微克/毫升）0、2、4、6、8和24小时。通过酶联免疫吸附测定法（ELISA）测量培养上清液中的细胞因子水平。使用反义细胞因子探针进行总RNA的Northern分析。
结果
脂多糖诱导培养24小时后的免疫反应性G-CSF和RANTES水平分别比未诱导细胞24小时的细胞因子水平高10倍和8.5倍（P < 0.01）。在脂多糖诱导下，G-CSF和RANTES转录的最大速率分别在24小时和8小时出现。
结论
孕早期滋养层细胞系中脂多糖诱导促炎细胞因子，这支持了孕早期滋养层细胞参与基于细胞因子的免疫信号传导以应对感染的观点。
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