Fujiwara S, Okuyama S, Imanaka T
Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Gene. 1996 Nov 7;179(1):165-70. doi: 10.1016/s0378-1119(96)00428-3.
Pyrococcus sp. KOD1 is a newly isolated hyperthermophilic archaeon from a solfatara at a wharf on Kodakara Island, Kagoshima, Japan. A physical map of the KOD1 chromosome was constructed using pulsed-field gel electrophoresis of restriction fragments generated by AscI, PacI and PmeI. The order of the AscI fragments was deduced from Southern hybridization using the AscI, PmeI and PacI fragments as a probe. The derived physical map indicates that KOD1 possesses a circular-form genome and its size was estimated to be 2036 kb. Several cloned genes were hybridized to restriction fragments to locate their positions on the physical map. Some genes involved in the central dogma were located on the restricted segment of the genome. Novel characteristics of KOD1 enzymes are also introduced in this article.
嗜热栖热菌属菌株KOD1是从日本鹿儿岛县甑岛一个码头的硫磺喷气孔中新分离出的嗜热古菌。利用AscI、PacI和PmeI产生的限制性片段进行脉冲场凝胶电泳，构建了KOD1染色体的物理图谱。AscI片段的顺序是通过以AscI、PmeI和PacI片段为探针的Southern杂交推导出来的。所得物理图谱表明KOD1拥有环状基因组，其大小估计为2036 kb。将几个克隆基因与限制性片段杂交，以确定它们在物理图谱上的位置。一些参与中心法则的基因位于基因组的限制片段上。本文还介绍了KOD1酶的新特性。
