Rudney J D, Ji Z, Larson C J
Department of Oral Science, School of Dentistry, University of Minnesota, Minneapolis 55455, USA.
J Dent Res. 1996 Oct;75(10):1789-97. doi: 10.1177/00220345960750101201.
Preliminary studies of 10 subjects suggested that saliva protein binding to oral bacteria might vary among oral sites. This study investigated saliva protein binding to layers of oral streptococci in an expanded sample of 48 subjects. Those persons were at opposite extremes for unstimulated whole saliva amylase, sIgA, lactoferrin, and lysozyme in an initial screening of 128 individuals. Layers of Streptococcus gordonii Blackburn or Streptococcus oralis 10557 on enamel chips were placed on buccal left and right upper premolars and molars (UL, UR), labial upper central incisors (UC), and lingual lower central incisors (LL). After a 10-minute exposure to saliva, bacterial extracts were assayed for bound amylase, sIgA, lactoferrin, and lysozyme. Those proteins also were quantified in unstimulated whole saliva collected after chip exposure. Both strains bound significantly more amylase at UL and UR, and significantly less at UC. Blackburn bound more amylase than 10557 at all sites. Significantly less sIgA was bound at UC; strain differences for sIgA were inconsistent across sites. Significantly more lactoferrin and lysozyme were bound at LL. There were no strain differences for lactoferrin; 10557 bound significantly more lysozyme at UL and UR. Subjects at opposite extremes for saliva protein concentrations differed for bound amylase and lactoferrin; those differences were smaller than site and strain differences. Bound protein levels were correlated across sites and strains. Correlations between whole saliva and bound proteins were moderate and were most consistent at LL. These findings suggest that saliva protein effects on oral ecology may vary among oral sites.
对10名受试者的初步研究表明，唾液蛋白与口腔细菌的结合可能因口腔部位而异。本研究在48名受试者的扩大样本中，调查了唾液蛋白与口腔链球菌层的结合情况。在对128名个体进行的初步筛查中，这些人的未刺激全唾液淀粉酶、分泌型免疫球蛋白A（sIgA）、乳铁蛋白和溶菌酶处于相反的极端水平。将牙釉质芯片上的戈登链球菌布莱克本菌株或口腔链球菌10557菌株放置在左侧和右侧上颌前磨牙及磨牙（UL、UR）、唇侧上颌中切牙（UC）和舌侧下颌中切牙（LL）处。在唾液中暴露10分钟后，对细菌提取物进行结合淀粉酶、sIgA、乳铁蛋白和溶菌酶的检测。这些蛋白质也在芯片暴露后收集的未刺激全唾液中进行定量。两种菌株在UL和UR处结合的淀粉酶明显更多，而在UC处明显更少。在所有部位，布莱克本菌株结合的淀粉酶都比10557菌株多。在UC处结合的sIgA明显更少；sIgA的菌株差异在不同部位不一致。在LL处结合的乳铁蛋白和溶菌酶明显更多。乳铁蛋白没有菌株差异；10557菌株在UL和UR处结合的溶菌酶明显更多。唾液蛋白浓度处于相反极端水平的受试者，其结合的淀粉酶和乳铁蛋白存在差异；这些差异小于部位和菌株差异。结合蛋白水平在不同部位和菌株之间存在相关性。全唾液与结合蛋白之间的相关性中等，在LL处最为一致。这些发现表明，唾液蛋白对口腔生态的影响可能因口腔部位而异。
