Arif Z, Ali R
Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A. M. U. Aligarh, India.
Biochem Mol Biol Int. 1996 Nov;40(5):871-9. doi: 10.1080/15216549600201483.
Native calf thymus DNA and poly(dA-dT).poly(dA-dT) were photo-adducted with 8-methoxypsoralen and characterized by thermal denaturation (Tm) and hydroxyapatite column chromatography. The data demonstrated the formation of interstrand photo-crosslinks. It has been shown by competition ELISA and band shift assays that crosslinked species of DNA-8-MOP and poly(dA-dT)-8-MOP photoadducts recognize previously defined monoclonal anti-Z-DNA antibody (Z22). The results indicate the possible presence of Z- or Z-like epitopes on nucleic acid-8-MOP crosslinks as Z22 antibody does not recognize other nucleic acid conformations. These studies also point out that conformational changes in DNA arising from the photo-addition could induce antibodies to DNA or could cause autoimmune disease.
天然小牛胸腺DNA和聚（dA-dT）·聚（dA-dT）用8-甲氧基补骨脂素进行光加合，并通过热变性（Tm）和羟基磷灰石柱色谱进行表征。数据表明形成了链间光交联。竞争ELISA和带移分析表明，DNA-8-MOP和聚（dA-dT）-8-MOP光加合物的交联物种可识别先前定义的抗Z-DNA单克隆抗体（Z22）。结果表明，核酸-8-MOP交联上可能存在Z或Z样表位，因为Z22抗体不能识别其他核酸构象。这些研究还指出，光加成引起的DNA构象变化可能诱导抗DNA抗体或导致自身免疫性疾病。
