Guido M E, Rusak B, Robertson H A
Department of Psychology, Dalhousie University, Halifax, Nova Scotia, Canada.
Brain Res. 1996 Nov 11;739(1-2):132-8. doi: 10.1016/s0006-8993(96)00816-5.
We have studied the expression of fosB mRNA in the suprachiasmatic nucleus (SCN) of hamsters by in situ hybridization using oligonucleotides with sequences complementary to the C-terminal of the fosB mRNA sequence. In animals exposed for 48 h to darkness, there was little or no background expression in SCN cells of fosB mRNA at any circadian phase. Light pulses (30 min) were able to induce fosB expression only during the subjective night. Transcripts of fosB increased rapidly to peak by the end of a 30-min light pulse. Light-induced increases gradually declined in darkness, but levels were still elevated for up to 150 min after the light pulse. Induction in response to a light pulse was largely restricted to the ventrolateral portion of the nucleus which receives the heaviest retinal projection. The temporal and anatomical pattern of fosB mRNA expression in the hamster SCN therefore resembles that reported previously for other immediate-early genes, such as c-fos.
我们使用与fosB mRNA序列C端互补的寡核苷酸，通过原位杂交研究了仓鼠视交叉上核（SCN）中fosB mRNA的表达。在暴露于黑暗48小时的动物中，在任何昼夜节律阶段，SCN细胞中fosB mRNA几乎没有背景表达。光脉冲（30分钟）仅在主观夜间能够诱导fosB表达。fosB的转录本在30分钟光脉冲结束时迅速增加至峰值。光诱导的增加在黑暗中逐渐下降，但在光脉冲后长达150分钟水平仍升高。对光脉冲的诱导主要局限于接受最密集视网膜投射的核的腹外侧部分。因此，仓鼠SCN中fosB mRNA表达的时间和解剖模式类似于先前报道的其他即刻早期基因，如c-fos。
