对抗生素反应改变的分枝杆菌属突变体的类固醇转化

Steroid transformation by mutants of Mycobacterium sp. with altered response to antibiotics.

作者信息

Barthakur S, Roy M K, Bera S K, Ghosh A C

机构信息

Division of Biochemistry, Regional Research Laboratory, Assam, India.

出版信息

J Basic Microbiol. 1996;36(6):383-7. doi: 10.1002/jobm.3620360602.

DOI:10.1002/jobm.3620360602

PMID:8956488

Abstract

Mycobacterium sp. NRRLB3683 which is capable to convert beta-sitosterol to 1,4-androstadiene-3,17-dione (ADD) was treated with methyl methane sulfonate and two strains with altered sensitivity to various antibiotics were obtained. One of the strain was steroid 1(2)-dehydrogenase negative and the other positive. Efficiency of utilization of sterols followed the order beta-sitosterol > cholesterol > soluble cholesterol. The steroid 1(2)-dehydrogenase negative strain was capable of producing 17KS (AD) from beta-sitosterol and converting AD to testosterone and ADD to AD suggesting the negative role of 1(2)-dehydrogenase in sterol side chain cleavage and decrease in hydrogenase activity by mutation. But this enzyme can perform the reverse reaction under aerobic condition.

摘要

能够将β-谷甾醇转化为1,4-雄甾二烯-3,17-二酮（ADD）的分枝杆菌属NRRLB3683用甲磺酸甲酯处理，获得了两株对各种抗生素敏感性改变的菌株。其中一株菌株类固醇1(2)-脱氢酶呈阴性，另一株呈阳性。甾醇的利用效率遵循β-谷甾醇>胆固醇>可溶性胆固醇的顺序。类固醇1(2)-脱氢酶阴性菌株能够从β-谷甾醇产生17KS（AD），并将AD转化为睾酮，将ADD转化为AD，这表明1(2)-脱氢酶在甾醇侧链裂解中起负作用，且突变导致氢化酶活性降低。但该酶在有氧条件下可进行逆反应。