Yokoi N, Hirano S, Okamoto S, Matsumoto Y, Yokoi K, Ikeda T, Kinoshita S, Katoh N, Yasuno H
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Am J Ophthalmol. 1996 Dec;122(6):825-9. doi: 10.1016/s0002-9394(14)70379-4.
To investigate the mechanism of atopic cataract development, we examined a tissue-destroying major basic protein, derived from eosinophils, in the anterior capsule of cataractous lenses and the aqueous fluid of atopic dermatitis patients.
Major basic protein deposition in anterior capsule tissues was evaluated immunohistochemically using anti-major basic protein monoclonal antibody in three consecutive eyes from three atopic cataract patients and three consecutive eyes from three senile cataract patients. Also, major basic protein concentration in aqueous fluid obtained during cataract surgery was measured by radioimmunoassay in 12 consecutive eyes from 11 atopic cataract patients and 15 consecutive eyes from 15 senile cataract patients.
Immunohistochemical investigation demonstrated lens epithelial major basic protein deposition in all three atopic cataracts but not in any of the senile cataracts. Major basic protein was detected in the aqueous fluid by radioimmunoassay in four of the 12 atopic cataract eyes and ranged in concentration from 11 to 70 ng/ml. No major basic protein was detected in the aqueous fluid of the 15 senile cataract eyes.
Major basic protein in anterior capsule tissues and in the aqueous fluid of eyes with atopic cataract may be associated with the pathogenesis of atopic cataract.
为了研究特应性白内障的发病机制，我们检测了来自嗜酸性粒细胞的一种破坏组织的主要碱性蛋白，该蛋白存在于特应性白内障患者的晶状体前囊膜及房水中。
使用抗主要碱性蛋白单克隆抗体，通过免疫组织化学方法评估3例特应性白内障患者的连续3只眼及3例老年性白内障患者的连续3只眼前囊膜组织中的主要碱性蛋白沉积情况。此外，采用放射免疫分析法检测11例特应性白内障患者的连续12只眼及15例老年性白内障患者的连续15只眼在白内障手术期间获取的房水中主要碱性蛋白的浓度。
免疫组织化学研究显示，所有3例特应性白内障均有晶状体上皮主要碱性蛋白沉积，而老年性白内障均未出现该情况。放射免疫分析法在12例特应性白内障眼中的4眼中检测到了主要碱性蛋白，其浓度范围为11至70 ng/ml。15例老年性白内障眼的房水中均未检测到主要碱性蛋白。
特应性白内障眼的前囊膜组织及房水中的主要碱性蛋白可能与特应性白内障的发病机制有关。
