Timofeeva O A, Peterson G M
Department of Anatomy and Cell Biology, East Carolina University School of Medicine, Greenville, NC 27858-4354, USA.
Epilepsy Res. 1996 Nov;25(3):149-59. doi: 10.1016/s0920-1211(96)00065-4.
The temporal/spatial dissemination of interictal spikes among different brain structures was studied during the course of kindling to determine if the long-term dissemination pattern reflects the rate and expression of kindling. The experiments were conducted on adult rabbits with chronically implanted electrodes (dorsal hippocampus, amygdala, caudate, all bilateral, sensory motor and occipital cortices). Rabbits (n = 13) were subjected to once daily electrical stimulation in the left hippocampus. Kindling resulted in the development of two different epileptic phenomena: 7 animals quickly (in 2-3 weeks) achieved a fully kindled state, characterized by generalized seizures, whereas the remaining 6 rabbits did not reliably progress beyond partial seizures even after more prolonged stimulation. Animals were accordingly divided into two groups referred to as generalized seizure and partial seizure. The temporal/spatial dissemination pattern, particularly in the two hippocampi, was very different between the groups. In both groups interictal spiking originated in one of the hippocampi independent of site stimulated and represented formation of the primary hippocampal epileptic focus. The generalized seizure group demonstrated stability of the primary hippocampal epileptic focus with permanent predominance of spiking in it over the course of kindling, and a high level of bilateral synchronous hippocampal interictal spiking. In the partial seizure group the primary hippocampal epileptic focus was established during the first 2-3 weeks of stimulation. This was later suppressed upon the development of an independent secondary focus in the opposite hippocampus. These animals also displayed very low levels of synchronous bilateral hippocampal interictal spiking. We suggest that an antagonistic relationship can develop between mirror hippocampal epileptic foci. This can be associated with a low level of bilateral hippocampal synchronous spiking, kindling retardation, and manifestation of partial seizures.
在点燃过程中，研究了发作间期棘波在不同脑结构间的时空传播，以确定长期传播模式是否反映点燃的速率和表现。实验在成年兔身上进行，这些兔长期植入电极（双侧背侧海马、杏仁核、尾状核、感觉运动皮层和枕叶皮层）。13只兔子每天接受一次左侧海马的电刺激。点燃导致两种不同癫痫现象的出现：7只动物迅速（2 - 3周内）达到完全点燃状态，其特征为全身性发作，而其余6只兔子即使在更长时间的刺激后也未能可靠地进展到部分发作以上。动物因此被分为两组，称为全身性发作组和部分发作组。两组之间的时空传播模式，特别是在两个海马中，非常不同。在两组中，发作间期棘波均起源于其中一个海马，与刺激部位无关，代表原发性海马癫痫灶的形成。全身性发作组显示原发性海马癫痫灶稳定，在点燃过程中其棘波始终占主导地位，且双侧海马发作间期棘波同步性较高。在部分发作组中，原发性海马癫痫灶在刺激的前2 - 3周内形成。随后，在对侧海马中出现独立的继发性病灶时，原发性病灶受到抑制。这些动物双侧海马发作间期棘波的同步性也非常低。我们认为，镜像海马癫痫灶之间可能会形成拮抗关系。这可能与双侧海马同步棘波水平低、点燃延迟以及部分发作的表现有关。
