Iotsova V, Crépieux P, Montpellier C, Laudet V, Stehelin D
Unité d'Oncologie Moléculaire, CNRS URA 1160, Institut Pasteur de Lille, France.
Oncogene. 1996 Dec 5;13(11):2331-7.
p53 has been reported to repress a number of TATA-containing promoters in transient transfection assays. TATA-less promoters are generally believed to be refractive to p53 repression. We report here that the TATA-less promoters of Ets-family genes (Ets-1 and Ets-2) are efficiently repressed by wild-type but not mutant p53 in transient co-transfection assays. Moreover, p53 was immunologically detected in protein complexes formed on oligonucleotides from both the TATA-containing and TATA-less promoters. Our data suggest that p53 is involved in the regulation of the expression of both promoter types, most probably by protein-protein interaction. A model for p53 function in promoter repression is proposed.
据报道，在瞬时转染实验中，p53可抑制许多含TATA框的启动子。一般认为，无TATA框的启动子对p53的抑制作用具有抗性。我们在此报告，在瞬时共转染实验中，Ets家族基因（Ets-1和Ets-2）的无TATA框启动子可被野生型而非突变型p53有效抑制。此外，在含TATA框和无TATA框启动子的寡核苷酸形成的蛋白质复合物中，通过免疫检测发现了p53。我们的数据表明，p53可能通过蛋白质-蛋白质相互作用参与这两种类型启动子表达的调控。本文提出了一个p53在启动子抑制中发挥作用的模型。
