Chuang Y H, Chiang B L, Chou C C, Hsieh K H
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, ROC.
Int Arch Allergy Immunol. 1996 Dec;111(4):366-71. doi: 10.1159/000237394.
It has been well documented that environmental factors such as antigen-presenting cells and related cytokines can affect the development of T-helper cells. The purpose of this study was to investigate the role of different antigen-presenting cells on T-cell development. Ovalbumin (OVA) combined with complete Freud's adjuvant (CFA) was used to sensitize mice subcutaneously or intraperitoneally, and then to follow-up production of IgG and IgE anti-OVA antibodies. In addition, semiquantitative PCR was used to determine the level of cytokine mRNA of different antigen-presenting cells. Resulting data showed that antigen-presenting cells expressed with different characteristics: (1) IgG2a anti-OVA antibody was higher in mice sensitized subcutaneously compared to those sensitized intraperitoneally. (2) The levels of cytokine mRNA were higher in antigen-stimulated spleen cells of mice immunized subcutaneously compared to those of mice immunized intraperitoneally. (3) Langerhans cells expressed a high level of IL-12; in contrast, peritoneal B cells expressed a high level of IL-10, but not IL-12. In summary, cytokine levels such as IL-10 and IL-12 were different among different kinds of APC, and their role in production of different isotypes of antibodies needs further elucidation.
已有充分文献记载，诸如抗原呈递细胞和相关细胞因子等环境因素会影响辅助性T细胞的发育。本研究的目的是调查不同抗原呈递细胞在T细胞发育中的作用。卵清蛋白（OVA）与完全弗氏佐剂（CFA）联合使用，对小鼠进行皮下或腹腔致敏，然后跟踪抗OVA抗体IgG和IgE的产生情况。此外，使用半定量PCR来确定不同抗原呈递细胞的细胞因子mRNA水平。所得数据表明，抗原呈递细胞具有不同的表达特征：（1）皮下致敏的小鼠中抗OVA抗体IgG2a高于腹腔致敏的小鼠。（2）皮下免疫小鼠的抗原刺激脾细胞中细胞因子mRNA水平高于腹腔免疫小鼠。（3）朗格汉斯细胞表达高水平的IL-12；相反，腹膜B细胞表达高水平的IL-10，但不表达IL-12。总之，不同种类的抗原呈递细胞中IL-10和IL-12等细胞因子水平不同，它们在不同同种型抗体产生中的作用需要进一步阐明。
