Profumo A, Profumo V, Vidali G
Laboratory of Molecular Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Electrophoresis. 1996 Oct;17(10):1617-21. doi: 10.1002/elps.1150171022.
Micellar electrokinetic chromatography (MEKC) using bile salts has been employed to separate retinoids differing in structure and charge; bile salts in MEKC allows the separation of liposoluble molecules but, to the best of our knowledge, there are only few data on the above-mentioned technique for the separation of highly hydrophobic compounds. The three natural vitamin A derivatives, retinal, retinol and retinoic acid, were successfully separated by MEKC using sodium cholate within a relatively short time (ca. 25 min), whereas the separation of these compounds was not successful using sodium dodecyl sulfate or sodium deoxycholate. Several parameters (pH and organic modifiers, in addition to bile salts concentration) have been tested to provide a system that can be extended to synthetic retinoids, which are often used in treating several diseases, including cancer prevention and therapy.
使用胆盐的胶束电动色谱法（MEKC）已被用于分离结构和电荷不同的类视黄醇；MEKC中的胆盐能够分离脂溶性分子，但据我们所知，关于上述分离高疏水性化合物技术的数据很少。三种天然维生素A衍生物，视黄醛、视黄醇和视黄酸，使用胆酸钠通过MEKC在相对较短的时间内（约25分钟）成功分离，而使用十二烷基硫酸钠或脱氧胆酸钠则无法成功分离这些化合物。已经测试了几个参数（除胆盐浓度外，还有pH值和有机改性剂），以提供一个可扩展到合成类视黄醇的系统，合成类视黄醇常用于治疗多种疾病，包括癌症预防和治疗。
