用胆酸钠通过胶束电动色谱法对手性多氯联苯进行拆分。
Chiral separation of polychlorinated biphenyls by micellar electrokinetic chromatography with sodium cholate.
作者信息
Crego A L, Gonzalez M J, Marina M L
机构信息
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Alcalá de Henares (Madrid), Spain.
出版信息
Electrophoresis. 1998 Sep;19(12):2113-8. doi: 10.1002/elps.1150191212.
Micellar electrokinetic chromatography (MEKC) with one kind of bile salt (sodium cholate) was used to separate three chiral polychlorinated biphenyls (PCBs; 84, 95, and 176), each one in its two enantiomers. Sodium cholate was used as chiral surfactant in a 2-(N-cyclohexylamino) ethanesulfonic acid (CHES) buffer under alkaline (pH 10) conditions containing urea (2 M). The influence of bile salt concentration on the efficiency and the resolution between the two enantiomers of PCBs 84 and 95 was established. The chiral separation of three PCBs was successfully achieved in less than 30 min (approximately 23 min for PCB 176 and approximately 29 min for PCBs 84 and 95).
采用含有一种胆盐（胆酸钠）的胶束电动色谱法（MEKC）分离三种手性多氯联苯（PCBs；84、95和176），每种多氯联苯的两种对映体均可分离。在含有尿素（2 M）的碱性（pH 10）2-（N-环己基氨基）乙磺酸（CHES）缓冲液中，胆酸钠用作手性表面活性剂。确定了胆盐浓度对PCBs 84和95两种对映体分离效率和分辨率的影响。三种PCBs的手性分离在不到30分钟内成功实现（PCB 176约23分钟，PCBs 84和95约29分钟）。
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