Iwasaki Y, Fujiike A, Kurita K, Ishihara K, Nakabayashi N
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
J Biomater Sci Polym Ed. 1996;8(2):91-102. doi: 10.1163/156856296x00174.
We evaluated the blood compatibility of various amphiphilic polymers, that is, n-butyl methacrylate (BMA) copolymers with methacrylates having a phosphorylcholine (PC), hydroxy (OH) or methoxy (MeO) group as an end polar group in the oxyethylene side chain. The amount of proteins adsorbed on the PC-polymer from human plasma was smaller than that on not only the poly(2-hydroxyethyl methacrylate) and poly(methyl methacrylate) but also the OH-polymer and MeO-polymer. The PC group could weaken the interaction between plasma proteins and polymer surfaces. The amount of adsorbed proteins on the PC-polymer decreased with an increase in the mole fraction of the PC units in the polymers. We could observe an effect of the oxyethylene chain length (n is the number of repeating units of oxyethylene) on protein adsorption between n = 2 and n = 3. The platelet adhesion on these polymer surfaces was evaluated using rabbit platelet-rich plasma. On the polymers without the PC group, that is, poly(BMA), OH-polymer, and MeO-polymer, many platelets adhered and a considerable shape change in the adherent platelets occurred. On the other hand, the PC-polymers could effectively suppress platelet adhesion. The platelet adhesion behavior on the polymers was strongly dependent on the adsorbed proteins. Platelet adhesion was completely inhibited on all of the PC-polymers studied having a 0.3 PC unit mole fraction. However, it was observed that the oxyethylene chains on the PC-polymers with a 0.1 PC unit mole fraction affected platelet adhesion.
我们评估了各种两亲性聚合物的血液相容性，即甲基丙烯酸正丁酯（BMA）共聚物，其在氧乙烯侧链中具有磷酰胆碱（PC）、羟基（OH）或甲氧基（MeO）基团作为末端极性基团的甲基丙烯酸酯。从人血浆中吸附在PC聚合物上的蛋白质数量不仅比聚（甲基丙烯酸2 - 羟乙酯）和聚（甲基丙烯酸甲酯）上的少，而且比OH聚合物和MeO聚合物上的少。PC基团可以减弱血浆蛋白与聚合物表面之间的相互作用。PC聚合物上吸附的蛋白质数量随着聚合物中PC单元摩尔分数的增加而减少。我们可以观察到氧乙烯链长度（n是氧乙烯的重复单元数）在n = 2和n = 3之间对蛋白质吸附的影响。使用富含兔血小板的血浆评估这些聚合物表面上的血小板粘附。在没有PC基团的聚合物上，即聚（BMA）、OH聚合物和MeO聚合物上，许多血小板粘附，并且粘附的血小板发生了相当大的形状变化。另一方面，PC聚合物可以有效抑制血小板粘附。聚合物上的血小板粘附行为强烈依赖于吸附的蛋白质。在所有研究的具有0.3 PC单元摩尔分数的PC聚合物上，血小板粘附被完全抑制。然而，观察到具有0.1 PC单元摩尔分数的PC聚合物上的氧乙烯链影响血小板粘附。
