Qu J, Chehroudi B, Brunette D M
Department of Oral Biology, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.
Oral Dis. 1996 Mar;2(1):102-15. doi: 10.1111/j.1601-0825.1996.tb00210.x.
Although currently available implants can be used to achieve osseointegration under well-defined conditions, a greater understanding of cell behaviour is required to improve the designs and embark on actual tissue engineering.
We employed micromachined substrata to investigate some of the main behavioural responses of osteoblasts from rat fetal calvaria to surface topography. In particular, confocal laser scanning microscopy (CLSM), differential interference contrast microscopy, time-lapse cinemicrography, immunofluorescence, digital radiography and image analysis were used to investigate cell adhesion, cell shape and cytoskeleton distribution, tissue organization, cell differentiation, and microenvironment.
A grooved surface permitted the attachment of more cells than a smooth one. Cell shape and cytoskeleton were strikingly influenced as early as 20 min after cell attachment, when the cytoskeleton begins to align with the topography. Some grooved surfaces appeared to promote osteogenesis in vitro as assessed by the production of bone-like nodules. Moreover, these nodules align with the topography in vitro, and preliminary results indicate that bone-like tissue also aligns with grooves when such surfaces are implanted in vivo.
尽管目前可用的植入物能够在明确的条件下实现骨整合，但仍需要更深入地了解细胞行为，以改进设计并开展实际的组织工程。
我们采用微加工基质来研究大鼠胎儿颅骨成骨细胞对表面形貌的一些主要行为反应。具体而言，利用共聚焦激光扫描显微镜（CLSM）、微分干涉相差显微镜、延时电影显微镜、免疫荧光、数字射线照相术和图像分析来研究细胞黏附、细胞形态和细胞骨架分布、组织构建、细胞分化以及微环境。
有凹槽的表面比光滑表面能附着更多细胞。早在细胞附着20分钟后，细胞形态和细胞骨架就受到显著影响，此时细胞骨架开始与形貌对齐。通过骨样结节的形成评估，一些有凹槽的表面似乎在体外促进了骨生成。此外，这些结节在体外与形貌对齐，初步结果表明，当将此类表面植入体内时，骨样组织也会与凹槽对齐。
