Wang Y, Yeh M
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
Immunology. 1996 Nov;89(3):316-23. doi: 10.1046/j.1365-2567.1996.d01-761.x.
We report here the cDNA cloning and variable region nucleic acid sequences of the heavy and the light chains of four monoclonal IgE and one monoclonal IgG1 antibodies against trichosanthin (TCS), all of which recognized a similar or related antigenic determinant. Sequence analysis revealed that the VH regions of the four IgE antibodies use different V gene segments from the 7183, 36-60 and J558 families. The DH genes varied extensively in nucleotide sequence and length, with D segments DSP2, DFL16 and DQ52 being used with equal frequency. All JH gene segments except JH1 were present in the epsilon transcripts. In contrast, there appeared to be restricted L-chain utilization. All four L-chain V genes cloned belonged to the V kappa 21 family and a bias in the use of J kappa 1 gene was also observed. The VL sequences of the anti-TCS monoclonal antibodies showed much less diversity than the VH sequences. We propose that the L-chain rather than H-chain may play a more significant role in specific binding with allergenic determinant on TCS.
我们在此报告针对天花粉蛋白（TCS）的四种单克隆IgE抗体和一种单克隆IgG1抗体的重链和轻链的cDNA克隆及可变区核酸序列，所有这些抗体均识别相似或相关的抗原决定簇。序列分析显示，四种IgE抗体的VH区使用来自7183、36 - 60和J558家族的不同V基因片段。DH基因在核苷酸序列和长度上差异很大，DSP2、DFL16和DQ52等D片段的使用频率相同。除JH1外，所有JH基因片段均存在于ε转录本中。相比之下，轻链的使用似乎受到限制。克隆的所有四条轻链V基因均属于Vκ21家族，并且还观察到Jκ1基因使用上的偏向性。抗TCS单克隆抗体的VL序列显示出的多样性远低于VH序列。我们提出，轻链而非重链可能在与TCS上的过敏原决定簇的特异性结合中发挥更重要的作用。
