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新型VH基因家族的成员存在于多克隆激活的B淋巴细胞的VDJ区域中。

Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.

作者信息

Winter E, Radbruch A, Krawinkel U

出版信息

EMBO J. 1985 Nov;4(11):2861-7. doi: 10.1002/j.1460-2075.1985.tb04015.x.

DOI:10.1002/j.1460-2075.1985.tb04015.x
PMID:2998759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554590/
Abstract

Four potentially productive and two non-productive VDJ gene segments were isolated from the DNA of mouse B-lymphocytes which had been polyclonally activated by bacterial lipopolysaccharide (LPS). Three VDJ regions exhibit VH genes which stem from two novel VH gene families. The complexity of these families is 5-9 genes. One of the non-productive VDJ regions exhibits a D segment which may have been generated by joining of two DSP2 segments. Both non-productive VDJ regions appear to contain rearranged pseudo VH genes. Three potential somatic mutations distributed over two productive VDJ regions are observed.

摘要

从经细菌脂多糖（LPS）多克隆激活的小鼠B淋巴细胞DNA中分离出4个潜在有功能和2个无功能的VDJ基因片段。三个VDJ区域表现出源自两个新的VH基因家族的VH基因。这些家族的复杂性为5 - 9个基因。其中一个无功能的VDJ区域表现出一个D片段，它可能是由两个DSP2片段连接产生的。两个无功能的VDJ区域似乎都包含重排的假VH基因。在两个有功能的VDJ区域观察到分布着三个潜在的体细胞突变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669f/554590/c8538b0befcf/emboj00276-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669f/554590/ad40ace0067d/emboj00276-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669f/554590/c8538b0befcf/emboj00276-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669f/554590/ad40ace0067d/emboj00276-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/669f/554590/c8538b0befcf/emboj00276-0144-a.jpg

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