Coates J P, Rowland S, Hill S, Iqball S, Bedford P A, Kimber I, Knight S C
Antigen Presentation Research Group, Imperial College of Science, Technology and Medicine, Northwick Park Institute, Harrow, Middlesex, UK.
Immunology. 1996 Nov;89(3):457-62. doi: 10.1046/j.1365-2567.1996.d01-746.x.
We compared the capacity of mature dendritic cells (DC) from lymph nodes and maturing DC from spleens in their capacity to stimulate responses to the small hapten picryl sulphonic acid (PIC) and to the same hapten conjugated to ovalbumin (PIC-OVA) and requiring processing. Surface expression of major histocompatibility complex (MHC) class II molecules, which are upregulated during maturation of splenic DC, were studied as an independent marker of maturation. Freshly isolated lymph node DC had a veiled appearance and high levels of class II expression. DC separated from suspensions of spleen cells expressed the DC-specific marker NLDC-145, but were small, had low levels of MHC class II molecules and expressed stem cell antigen. Those DC from spleen cells cultured for 24 and 48 hr showed the development of typical veiled DC morphology and high class II expression. Lymph node DC stimulated high levels of primary T-cell proliferation to PIC, but failed to stimulate primary responses to PIC-OVA. Splenic DC isolated immediately failed to stimulate primary responses to either antigen. More mature spleen DC stimulated responses both to PIC and PIC-OVA. Surprisingly, development of the capacity to stimulate responses to PIC preceded that of stimulating PIC-OVA responses. The capacity of the DC to process and present PIC-OVA was maintained during the culture period. The results indicate that both the form of the antigen and the source and maturity of the DC are critical in determining the responses stimulated in T lymphocytes.
我们比较了来自淋巴结的成熟树突状细胞（DC）和来自脾脏的正在成熟的DC刺激对半抗原苦味酸磺酸（PIC）以及与卵清蛋白偶联的同一半抗原（PIC-OVA，需要加工处理）产生反应的能力。作为成熟的独立标志物，研究了主要组织相容性复合体（MHC）II类分子的表面表达情况，其在脾脏DC成熟过程中上调。新鲜分离的淋巴结DC呈面纱样外观，II类分子表达水平高。从脾细胞悬液中分离出的DC表达DC特异性标志物NLDC-145，但体积小，MHC II类分子水平低且表达干细胞抗原。那些培养24小时和48小时的脾细胞来源的DC呈现出典型的面纱样DC形态且II类分子高表达。淋巴结DC刺激对PIC的高水平初始T细胞增殖，但未能刺激对PIC-OVA的初始反应。立即分离出的脾DC未能刺激对任何一种抗原的初始反应。更成熟的脾DC刺激对PIC和PIC-OVA的反应。令人惊讶的是，刺激对PIC反应的能力的发展先于刺激对PIC-OVA反应的能力的发展。在培养期间，DC加工和呈递PIC-OVA的能力得以维持。结果表明，抗原的形式以及DC的来源和成熟度在决定T淋巴细胞所激发的反应中至关重要。
