Hill S, Coates J P, Kimber I, Knight S C
Antigen Presentation Research Group, St Mary's Hospital Medical School, Northwick Park Hospital, Harrow, UK.
Immunology. 1994 Oct;83(2):295-301.
Dendritic cells (DC) isolated from the lymph nodes or spleens of mice and pulsed with contact sensitizers or protein antigens stimulate primary proliferative responses by syngeneic T cells and responses to alloantigens in the mixed leucocyte reaction (MLR). Using enriched human peripheral blood DC, we attempted to stimulate primary immune responses to contact sensitizers by autologous lymphocytes in vitro. No significant proliferation above background levels or CD69 expression (an early activation antigen on lymphocytes) was detected despite using a wide range of donors, chemicals, antigens and cell concentrations. Culture of DC for up to 5 days in vitro in the presence of phytohaemagglutinin (PHA)-conditioned culture supernatants, or recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) also failed to induce primary proliferative responses to contact sensitizers. Comparisons were made between blood and lymph node DC from mice to explore whether the lack of stimulation was the result of differences between mouse and human DC or between DC isolated from different tissues. DC from lymph nodes stimulated primary responses to contact sensitizers in both blood and lymph node lymphocytes whereas blood DC did not stimulate responses. Both lymph node and blood DC stimulated an allogeneic MLR, although blood DC were less efficient than those from lymph node. The data show that DC from different tissues exhibit variable functional activity. DC from blood and lymph nodes were examined to determine whether surface antigen expression is related to functional activity. Murine blood DC expressed similar levels of LFA-1, LECAM-1 and CD44 compared with lymph node DC but lower levels of MHC class II, B7 and ICAM-1. These results may therefore have important implications for antigen processing and presentation in cells from different tissue compartments.
从小鼠淋巴结或脾脏中分离出的树突状细胞（DC），经接触性致敏剂或蛋白质抗原刺激后，可激发同基因T细胞的原发性增殖反应以及混合淋巴细胞反应（MLR）中对同种异体抗原的反应。我们利用富集的人外周血DC，试图在体外刺激自体淋巴细胞对接触性致敏剂产生原发性免疫反应。尽管使用了广泛的供体、化学物质、抗原和细胞浓度，但未检测到高于背景水平的显著增殖或CD69表达（淋巴细胞上的一种早期激活抗原）。在植物血凝素（PHA）预处理的培养上清液或重组粒细胞-巨噬细胞集落刺激因子（GM-CSF）存在的情况下，将DC在体外培养长达5天，也未能诱导对接触性致敏剂的原发性增殖反应。对小鼠血液和淋巴结DC进行了比较，以探讨缺乏刺激是小鼠和人DC之间差异的结果，还是不同组织分离出的DC之间差异的结果。淋巴结DC可刺激血液和淋巴结淋巴细胞对接触性致敏剂产生原发性反应，而血液DC则不能刺激反应。淋巴结和血液DC均可刺激同种异体MLR，尽管血液DC的效率低于淋巴结DC。数据表明，不同组织的DC表现出可变的功能活性。对血液和淋巴结DC进行检测，以确定表面抗原表达是否与功能活性相关。与淋巴结DC相比，小鼠血液DC表达的淋巴细胞功能相关抗原-1（LFA-1）、白细胞内皮细胞黏附分子-1（LECAM-1）和CD44水平相似，但主要组织相容性复合体II类（MHC class II）、B7和细胞间黏附分子-1（ICAM-1）水平较低。因此，这些结果可能对不同组织区室细胞中的抗原加工和呈递具有重要意义。
