Thorsen K, Kristoffersson A O, Lerner U H, Lorentzon R P
Department of Orthopedics, University of Umeå, Sweden.
J Clin Invest. 1996 Dec 1;98(11):2446-9. doi: 10.1172/JCI119061.
In this study we describe, to our knowledge, the first experiment using the microdialysis technique for studying the release of prostaglandin E2 (PGE2) in the proximal tibia metaphysis secondary to mechanical loading. Nine healthy females, six in the loading group and three controls, mean age 34+/-2 (years+/-SEM), participated. A standard microdialysis catheter was inserted into the tibia metaphyseal bone under local anesthesia. Samplings were done every 15 min under a 2-h equilibration period. Thereafter, heel-drops (one impact per second) with as hard impact of the heels into the floor as possible, were done for 5 min by the subjects in the loading group. The control group performed no exercise. Sampling continued after this for another 2-h period. Basal levels of PGE2 in the proximal tibial metaphysis were determined to a mean of 45+/-10 pg/ml (mean+/-SEM, n = 6). The major finding was a 2.5-3.5-fold increase in the release of PGE2 after mechanical loading. The increase was statistically significant (P < 0.05 compared with basal levels) 1 h after the mechanical loading and persisted for the rest of the experimental period. No major alterations were observed in the control group. In conclusion, our data demonstrate that in situ microdialysis is a useful method for studying the PGE2 production in human bone. Furthermore, a rapid and significant increase in PGE2 levels was noticed in response to dynamic mechanical loading.
据我们所知，在本研究中，我们描述了首个使用微透析技术研究机械负荷继发于胫骨近端干骺端中前列腺素E2（PGE2）释放的实验。九名健康女性参与其中，六名在负荷组，三名作为对照组，平均年龄34±2（岁±标准误）。在局部麻醉下将标准微透析导管插入胫骨干骺端骨。在2小时的平衡期内每隔15分钟进行一次采样。此后，负荷组的受试者进行足跟下落（每秒一次冲击），尽可能用力地让足跟撞击地面，持续5分钟。对照组不进行运动。此后采样再持续2小时。测定胫骨近端干骺端PGE2的基础水平，平均为45±10 pg/ml（平均值±标准误，n = 6）。主要发现是机械负荷后PGE2的释放增加了2.5至3.5倍。机械负荷后1小时，这种增加具有统计学意义（与基础水平相比，P < 0.05），并在实验期的剩余时间持续存在。对照组未观察到重大变化。总之，我们的数据表明，原位微透析是研究人体骨骼中PGE2产生的一种有用方法。此外，发现PGE2水平响应动态机械负荷迅速且显著增加。
