Wuthiekanun V, Smith M D, Dance D A, Walsh A L, Pitt T L, White N J
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J Med Microbiol. 1996 Dec;45(6):408-12. doi: 10.1099/00222615-45-6-408.
The biochemical characteristics of 213 isolates of Burkholderia pseudomallei from patients with melioidosis and 140 isolates from the soil in central and northeastern Thailand were compared. Whereas the biochemical profiles of all the clinical isolates were similar, all soil isolates from the central area and 25% of isolates from northeastern Thailand comprised a different phenotype. This was characterised by the ability to assimilate L-arabinose (100%), adonitol (100%), 5-keto-gluconate (90%) and D-xylose (84%), but failure to assimilate dulcitol (0%), erythritol (0%) and trehalose (10%). Compared with clinical isolates, these organisms had similar antibiotic susceptibility profiles and were also recognised by a specific polyclonal antibody against B. pseudomallei. As melioidosis is rare in central Thailand, but common in the northeast, this raises the possibility that this biochemical phenotype may be less virulent, or may even represent a different species.
对213株来自类鼻疽患者的伯克霍尔德菌和140株来自泰国中部及东北部土壤的伯克霍尔德菌的生化特性进行了比较。所有临床分离株的生化谱相似，而中部地区的所有土壤分离株以及泰国东北部25%的分离株呈现出不同的表型。其特征为能够同化L-阿拉伯糖（100%）、阿东醇（100%）、5-酮葡萄糖酸盐（90%）和D-木糖（84%），但不能同化卫矛醇（0%）、赤藓醇（0%）和海藻糖（10%）。与临床分离株相比，这些菌株具有相似的抗生素敏感性谱，并且也能被针对类鼻疽伯克霍尔德菌的特异性多克隆抗体识别。由于类鼻疽在泰国中部罕见，但在东北部常见，这增加了这种生化表型可能毒力较低甚至可能代表不同物种的可能性。
