Pathogen and Microbiome Institute, Northern Arizona Universitygrid.261120.6, Flagstaff, Arizona, USA.
Discipline of Biomedicine and Australian Institute of Tropical Health and Medicine, James Cook Universitygrid.1011.1, Townsville, Queensland, Australia.
Appl Environ Microbiol. 2022 Jan 11;88(1):e0158321. doi: 10.1128/AEM.01583-21. Epub 2021 Oct 13.
Distinct strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to sp. nov. include type strain BDU6 (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to sp. nov. include type strain MSMB266 (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both sp. nov. and sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within sp. nov. and sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a sp. nov. cluster separate from other species, whereas sp. nov. strains did not form a distinct cluster. Neither sp. nov. nor sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of sp. nov. and sp. nov. results in a total of eight species currently within the Bpc. species can be important sources of novel natural products, and new species are of interest to diverse scientific disciplines. Although many species are saprophytic, Burkholderia pseudomallei is the causative agent of the disease melioidosis. Understanding the genomics and virulence of the closest relatives to B. pseudomallei, i.e., the other species within the B. pseudomallei complex (Bpc), is important for identifying robust diagnostic targets specific to B. pseudomallei and for understanding the evolution of virulence in B. pseudomallei. Two proposed novel species, sp. nov. and sp. nov., were isolated from soil samples collected from multiple locations in northern Australia. The two proposed species belong to the Bpc but are phylogenetically distinct from all other members of this complex. The addition of sp. nov. and sp. nov. results in a total of eight species within this significant complex of bacteria that are available for future studies.
从澳大利亚北部热带地区（北领地和托雷斯海峡群岛、昆士兰州）采集的土壤样本中分离出了不同的菌株。16S rRNA 和全基因组序列的系统发育分析表明，这些菌株与先前描述的种不同，并将它们分配到伯克霍尔德氏菌复合体（Bpc）内的两个新分支中。由于平均核苷酸同一性和数字 DNA-DNA 杂交计算结果一致，表明这些分支代表不同的种，因此我们提议将 Burkholderia mayonis sp. nov. 和 Burkholderia savannae sp. nov. 命名为 Burkholderia mayonis sp. nov. 和 Burkholderia savannae sp. nov. 。属于 sp. nov. 的菌株包括模式菌株 BDU6（=TSD-80；LMG 29941；ASM152374v2）和 BDU8。属于 sp. nov. 的菌株包括模式菌株 MSMB266（=TSD-82；LMG 29940；ASM152444v2）、MSMB852、BDU18 和 BDU19。比较基因组学揭示了这两个假定种的独特编码区域，包括与噬菌体相关的同源基因簇。这两个新种的模式菌株在生化特征上彼此不同，与 Bpc 中的其他种也不同，而且在 sp. nov. 和 sp. nov. 中的菌株之间也存在差异。基质辅助激光解吸电离飞行时间（MALDI-TOF）分析显示，一个 sp. nov. 聚类与其他种分开，而 sp. nov. 菌株则没有形成一个独特的聚类。sp. nov. 和 sp. nov. 都不会导致通过皮下途径给药的小鼠死亡。增加 sp. nov. 和 sp. nov. 后，Bpc 中共有 8 个种。种是新型天然产物的重要来源，新种是许多科学学科感兴趣的对象。尽管许多种是腐生的，但伯克霍尔德氏菌假单胞菌是类鼻疽病的病原体。了解与伯克霍尔德氏菌假单胞菌最接近的亲缘种（即伯克霍尔德氏菌复合体（Bpc）中的其他种）的基因组学和毒力对于确定针对伯克霍尔德氏菌假单胞菌的稳健诊断靶标以及了解伯克霍尔德氏菌假单胞菌毒力的进化非常重要。两个拟议的新种，sp. nov. 和 sp. nov. 是从澳大利亚北部多个地点采集的土壤样本中分离出来的。这两个拟议的种属于 Bpc，但在系统发育上与该复合体的所有其他成员都不同。增加 sp. nov. 和 sp. nov. 后，该重要细菌复合体共有 8 个种可供未来研究。
